nsLTPDB - the non-specific Lipid Transfer Protein Database for Plant

Welcome to the nsLTPDB

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Plant nonspecific lipid transfer proteins (nsLTPs) have been purified from various sources such as wheat, maize, apricot, rice, and others.(Poznanski et al., 1999) They contain sp-LTP and ns-LTP . The sp-LTP which are acidic 20- to 30- KDa proteins and are specific only for one or two classes of phospholipid; the ns-LTP which are basic 7- to 15- KDa proteins and possess broad specificity for several classes of phospholipid and/or glycolipids.

The two main group of ns-LTPs, ns-LTP1 and ns-LTP2 , have been identified with molecular masses of about 9 and 7 KDa . ( Protein Sci. 5 (1996) 565-577. )Homologies between amino acid sequences range from 70% to 30% among plant LTP, but they contain eight cysteine residues in the sequence were completely conserved, and form 4 disulfide bonds . The structure of LTP has shown that these proteins share a common fold, stabilized by four disulfide bonds, and are characterized by a four helix bundle covered partly by a long C-terminal arm with several tures. The three-dimensional fold of nsLTP is composed of four Alpha helices and a flexible stretch of C-terminal residues. ( Samuel et al., 2002; Pons et al., 2003)

Different functions are reported for LTPs: transport of hydrophobic molecules such as:
Phospholipids between membranes (Tree Physiol. 29 (2009) 1607-1619.), Cutin formation Arch. (Biochem. Biophys. 311 (1994) 460-468.) Protection against pathogens (J. Biol. Chem. 267 (1992) 15301-15309.)

The nsLTPDB provides some informations for studying Plant and Lipid Transfer Protein relationships. This site also offers some analysis results like Phylogenetic tree and Multiple sequence alignment in order to offer some information in academic researches and biotechnological developments.



Primary data of nsLTPDB were collected from the National Center for Biotechnology Information (NCBI) and related plant database by using program and visual inspections were then performed to refine the data.



The non - specific Lipid Transfer Protein subset of nsLTPDB contains about 121 species.



Our Lab's References

Liu YJ, Samuel D, Lin CH, Lyu PC.: Purification and characterization of a novel 7-kDa non-specific lipid transfer protein-2 from rice (Oryza sativa).Biochem Biophys Res Commun 2002, Jun 14;294(3):535-40. [Abstract] [PDF]

Samuel D, Liu YJ, Cheng CS, Lyu PC.: Solution structure of plant nonspecific lipid transfer protein-2 from rice (Oryza sativa). J Biol Chem. 2002 Sep 20;277(38):35267-73. [Abstract] [PDF]

Cheng HC, Cheng PT, Peng P, Lyu PC, Sun YJ.: Lipid binding in rice nonspecific lipid transfer protein-1 complexes from Oryza sativa. Protein Sci. 2004 Sep;13(9):2304-15. Epub 2004 Aug 4.[Abstract] [PDF]

Cheng CS, Samuel D, Liu YJ, Shyu JC, Lai SM, Lin KF, Lyu PC.:Binding mechanism of nonspecific lipid transfer proteins and their role in plant defense.Biochemistry. 2004 Nov 2;43(43):13628-36.[Abstract] [PDF]

Lin KF, Liu YN, Hsu ST, Samuel D, Cheng CS, Bonvin AM, Lyu PC.: Characterization and structural analyses of nonspecific lipid transfer protein 1 from mung bean. Biochemistry. 2005 Apr 19;44(15):5703-12., 58:618-627.[Abstract] [PDF]

Cheng CS, Chen MN, Lai YT, Chen T, Lin KF, Liu YJ, Lyu PC.:Mutagenesis study of rice nonspecific lipid transfer protein 2 reveals residues that contribute to structure and ligand binding.Proteins. 2008 Feb 15;70(3):695-706.[Abstract] [PDF]

Lai YT, Cheng CS, Liu YN, Liu YJ, Lyu PC.:Effects of ligand binding on the dynamics of rice nonspecific lipid transfer protein 1: a model from molecular simulations. Proteins. 2008 Sep;72(4):1189-98.[Abstract] [PDF]


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